Metal chelate affinity chromatography of hamster interferon
- 1 June 1978
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 58 (2) , 149-152
- https://doi.org/10.1007/bf01315407
Abstract
Summary Syrian hamster interferon, produced by benzo (a) pyrene-transformed embryo cells does not display an affinity for Zn++ chelate-agarose. However, the interferon binds to Cu++ chelate-agarose and can be displaced from the column by a falling pH-gradient. The chromatography of crude hamster interferon preparation on a tandem of columns: Zn++ chelate-agarose → Cu++ chelate-agarose results in a 25-fold purification of interferon.Keywords
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