Analysis of Cellular Phenotypes That Mediate Genetic Resistance to Tuberculosis Using a Radiation Bone Marrow Chimera Approach

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Abstract
Adoptive transfer of bone marrow cells from tuberculosis-resistant (I/St × A/Sn)F1donor mice into lethally irradiated susceptible I/St recipients changed their phenotype following infection with virulentMycobacterium tuberculosis. Compared to I/St→I/St control animals, F1→I/St chimeras demonstrated (i) prolonged survival time, (ii) increased antimycobacterial function of lung macrophages, (iii) elevated gamma interferon production by lung cells, and (iv) decreased infiltration of the lungs with CD4+and CD8+T cells and Ly-6G+neutrophils.