In Vitro Culture, Storage and Transfer of Goat Embryos

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Abstract
Goat embryos, collected 5 and 7 days after mating, were cultured in vitro at 37.degree. C for 2 days, or stored at 5.degree. C for 1 or 2 days and then cultured for 2 days, or stored in liquid N (-196.degree. C) for 2-4 wk and then cultured for 1 day. After culture some of the embryos were transferred to recipient doses. Culture and storage was carried out in Dulbecco phosphate buffer enriched with 25% goat serum; 1M glycerol or 2M dimethylsulfoxide was added to the media used for frozen storage. Of 15 embryos 13 cultured without prior storage showed apparently normal development in culture, of the Se 10 were transferred and 5 kids were born. Twenty of 38 embryos stored at 5.degree. C developed in culture and 6 kids were born following the transfer of 17 embryos. Duration of storage at 5.degree. C had no marked effect upon subsequent development. Six of 48 frozen stored embryos development in culture. All 6 were transferred and 3 kids were born.

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