Rapid determination of the susceptibility of bacteria to antibiotics with ‘Sensititre’ plates and nitroblue tetrazolium
- 1 November 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 8 (5) , 363-369
- https://doi.org/10.1093/jac/8.5.363
Abstract
A rapid (4 h), reliable and technically simple method for determination of the minimum inhibitory concentration of antibiotics for bacteria using ‘Sensititre’ plates and nitroblue tetrazolium (NBT) for early detection of bacterial growth is described. MIC-values obtained with the rapid NBT method for 70 isolates from urine were compared with those obtained with Sensititre plates incubated overnight. The results were within one two-fold dilution in 80–100% of the cases depending on the species. As to the reproducibility of the NBT method, the same degree of agreement was obtained in 98% of the cases when determinations on 7 strains against 9 or 10 antibiotics were repeated on 10 different occasions.Keywords
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