Analysis of the Water Permeability of Human Granulocytes at Subzero Temperatures in the Presence of Extracellular Ice
- 1 November 1983
- journal article
- Published by ASME International in Journal of Biomechanical Engineering
- Vol. 105 (4) , 360-366
- https://doi.org/10.1115/1.3138433
Abstract
The plasma membrane water permeability of human granulocytes in the presence of extracellular ice was determined experimentally on a cryomicroscope. Transient volumes of individual cells were measured at constant subzero temperatures subsequent to ice nucleation. Permeability values were deduced by adjustment of multiple parameters in a model to obtain an optimal fit to the data. The permeability was determined to be a function of both temperature and intracellular solute osmolality, with a reference value at 0°C of 0.407 μm/atm·min and temperature and solute coefficients of 218kJ/mol and 1.09 Osm/kg.Keywords
This publication has 0 references indexed in Scilit: