Further studies on section thickness measurement
- 1 July 1976
- journal article
- research article
- Published by Springer Nature in Journal of Molecular Histology
- Vol. 8 (4) , 383-386
- https://doi.org/10.1007/bf01003827
Abstract
A rapid, simple, accurate and reproducible technique for measuring section thickness using an instrument known as a surfometer is described. It has shown that microtome settings cannot be relied upon as a means of monitoring the thickness of sections of quenched or wax-embedded tissue. However, there appeared to be a linear relationship between microtome setting and the thickness of dewaxed sections. Microtome settings seem to provide an accurate indication of the thickness of araldite sections.Keywords
This publication has 3 references indexed in Scilit:
- Measurement of section thickness in quantitative microscopy with special reference to enzyme histochemistryJournal of Clinical Pathology, 1974
- The chemical determination of section thicknessHistochemistry and Cell Biology, 1971
- Studies on thickness variation in ultrathin sections for electron microscopyJournal of the Royal Microscopical Society, 1966