Photooxidation of NADH by 2,3-butanedione: a potential source of error in studies on active site arginyl residues
- 1 June 1979
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 57 (6) , 977-979
- https://doi.org/10.1139/o79-119
Abstract
Incubation of NADH or NADPH with 2,3-butanedione in aqueous solution results in photooxidation of the reduced pyridine nucleotides under conditions of ordinary laboratory lighting. Maximum rates of photooxidation are obtained at pH 7 and with light at a wavelength of 410 nm. This reaction could lead to artifactual results in experiments on the role of arginyl groups in enzymes in which a reduced pyridine nucleotide is used to protect the active site residues from modification by 2,3-butanedione.This publication has 2 references indexed in Scilit:
- Involvement of an essential arginyl residue in the coupling activity of Rhodospirillum rubrum chromatophoresArchives of Biochemistry and Biophysics, 1978
- Modification of ferredoxin-NADP+ reductase from the alga Bumilleriopsis with butanedione and dansyl chlorideArchives of Biochemistry and Biophysics, 1978