Evidence for an Adaptive Response to Radiation Damage in Plant Cells Conditioned with X-rays or Incorporated Tritium

Abstract
Allium cepa root-tip cells were first exposed to low ‘conditioning’ doses of ionizing radiation: to X-rays (0·06 or 0·26 Gy) or to incorporated tritium (1·8 × 104 or 7·2 × 104 Bq/ml; specific activity: 740·0 GBq/mmol) and subsequently given a ‘challenge’ dose of 1·5 Gy of X-rays. A reduction in X-ray-induced chromosomal damage was brought about by prior exposure to 0·26 Gy of X-rays, while cells receiving the lower conditioning dose (0·06 Gy of X-rays) did not show any significant reduction. In cells grown in the presence of [3H]TdR on the other hand, the adaptive response was evident after both doses given. The results are essentially in agreement with those published by Wolff's group for human lymphocytes in showing that plant cells in vivo can become ‘adapted’ by exposure to low-level irradiation so that they become more resistant to the clastogenic effects of X-rays delivered subsequently.