Drosophila RNAi Screen Reveals CD36 Family Member Required for Mycobacterial Infection
Top Cited Papers
- 19 August 2005
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 309 (5738) , 1251-1253
- https://doi.org/10.1126/science.1116006
Abstract
Certain pathogens, such as Mycobacterium tuberculosis , survive within the hostile intracellular environment of a macrophage. To identify host factors required for mycobacterial entry and survival within macrophages, we performed a genomewide RNA interference screen in Drosophila macrophage-like cells, using Mycobacterium fortuitum . We identified factors required for general phagocytosis, as well as those needed specifically for mycobacterial infection. One specific factor, Peste (Pes), is a CD36 family member required for uptake of mycobacteria, but not Escherichia coli or Staphylococcus aureus . Moreover, mammalian class B scavenger receptors (SRs) conferred uptake of bacteria into nonphagocytic cells, with SR-BI and SR-BII uniquely mediating uptake of M. fortuitum , which suggests a conserved role for class B SRs in pattern recognition and innate immunity.Keywords
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