Cleavage Analysis of Actinophage R4 and Its Deletion Mutants
- 1 August 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 47 (8) , 1873-1878
- https://doi.org/10.1080/00021369.1983.10865878
Abstract
We have constructed restriction maps of actinophage R4 for PstI, PvuII, EcoRI, SeaI, KpnI, BglII and BclI. DNA of R4 phage had no cleavage sites for seven restriction endonucleases, BamHI, ClaI, HindIII, SacII, SlaI (XhoI) or XbaI. The entire genome size of the R4 phage was estimated to be 53.7 kilobases. Three deletions of the R4 phage were delimitated on the R4 genome. All three deletions were located on the right hand side of the R4 restriction map.This publication has 2 references indexed in Scilit:
- A DNA cloning system for interspecies gene transfer in antibiotic-producing StreptomycesNature, 1980
- VIABLE T4 BACTERIOPHAGE CONTAINING CYTOSINE- SUBSTITUTED DNA (T4dC PHAGE)The Journal of General and Applied Microbiology, 1978