Isolation of Basic Acrosin Inhibitor from Bull Seminal Plasma (BUSI II)
- 1 January 1979
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 360 (2) , 1753-1758
- https://doi.org/10.1515/bchm2.1979.360.2.1753
Abstract
An acrosin inhibitor was isolated from bull seminal plasma by gel filtration on Sephadex G-50 fine and ion-exchange chromatography on CM-Sephadex. The inhibitor is a basic polypeptide (pI [isoelectric point] .gtoreq. 10.5) of MW 6200 (calculated from amino acid composition). Its N-terminal amino group is blocked. The inhibitor is not strictly specific in its effect since it also inhibits trypsin [EC 3.4.21.4] and to a lesser degree chymotrypsin [EC 3.4.21.1], in addition to bull and boar acrosin.This publication has 9 references indexed in Scilit:
- Characterization of the Proteinase Inhibitors from Bull Seminal Plasma and SpermatozoaHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1976
- Biochemistry of Mammalian FertilizationAnnual Review of Biochemistry, 1974
- The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1969
- High recovery of tryptophan from acid hydrolysates of proteinsBiochemical and Biophysical Research Communications, 1969
- Über einen hormonabhängigen Inhibitor für proteolytische Enzyme in männlichen accessorischen Geschlechtsdrüsen und im SpermaHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1966
- Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. IV. Further Studies on the Resolving Power in Connection with Separation of Myoglobins.Acta Chemica Scandinavica, 1966
- Aminosäure-p-nitroanilide als Substrate für Aminopeptidasen und andere proteolytische FermenteHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1962
- THE OXIDATION OF RIBONUCLEASE WITH PERFORMIC ACIDJournal of Biological Chemistry, 1956
- A spectrophotometric determination of trypsin and chymotrypsinBiochimica et Biophysica Acta, 1955