Genetic Complementation of the Immortal Phenotype in Group D Cell Lines by Introduction of Chromosome 7

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Abstract
Human immortal cell lines have been classified into at least four (A–D) genetic complementation groups by cell‐cell hybrid analysis, i.e., a hybrid derived from different groups becomes mortal. Recently we have demonstrated that introduction of human chromosome 7 suppresses indefinite division potential in the non‐tumorigenic human immortalized fibroblast lines KMST‐6 and SUSM‐1, both assigned to complementation group D. By extending our microcell‐mediated chromosome transfer, we found that chromosome 7 also suppresses division potential in the human hepatoma line HepG2 (again, assigned to group D). Chromosome 7 was thus shown to suppress indefinite growth in the above group D cell lines irrespective of their cell types, or whether they are tumorigenic or not. Since chromosome 7 had no such effect on representative cell lines derived from complementation group A, B or C, these results indicate that the senescence gene(s) commonly mutated in the group D cell lines is located on chromosome 7.