CHARACTERIZATION OF A PHOSPHOGLYCERATE KINASE-DEFICIENCY VARIANTS NOT ASSOCIATED WITH HEMOLYTIC-ANEMIA

  • 1 January 1980
    • journal article
    • research article
    • Vol. 32  (3) , 364-373
Abstract
The properties of a variant phosphoglycerate kinase (PGK) found in a large German clan were examined. The normal and variant enzymes, isolated by affinity chromatography, have the same MW, specific activity, substrate affinity and nearly identical pH optima. Using immunoinactivation and immunodiffusion, the same specific activity for both forms was again determined. Since the enzymatic activity in older and younger erythrocytes varied only slightly, and since the specific activity of the variant was normal, the variant seems to be stable in vivo. The decreased enzyme content may be due to a decreased synthesis rate. The variant PGK described here is distinctly different from the known PGK variants and was designated PGK Muenchen.