Increased nuclear cyclin/PCNA antigen staining of non S‐phase transformed human amnion cells engaged in nucleotide excision DNA repair
- 15 December 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 209 (2) , 277-283
- https://doi.org/10.1016/0014-5793(86)81127-9
Abstract
PCNA autoantibodies specific for cyclin/PCNA were used to determine the nuclear distribution of this protein in transformed human amnion cells (AMA) irradiated with ultraviolet light (254 nm) under conditions that induced nucleotide excision DNA repair synthesis. The results showed a striking increase in nuclear cyclin/PCNA antigen staining of non S-phase cells that was not abolished by cycloheximide (20 micrograms/ml, added 2 h before irradiation), and that is most likely due to a redistribution of pre-existing cyclin. These observations raise the possibility that cyclin/PCNA may play a role in nucleotide excision DNA repair synthesis in addition to its putative role in replicative DNA synthesis.Keywords
This publication has 22 references indexed in Scilit:
- Structural organizations of replicon domains during DNA synthetic phase in the mammalian nucleusExperimental Cell Research, 1986
- Synthesis of the nuclear protein cyclin (PCNA) and its relationship with DNA replicationExperimental Cell Research, 1986
- S‐phase patterns of cyclin (PCNA) antigen staining resemble topographical patterns of DNA synthesisFEBS Letters, 1985
- Changes in the nuclear distribution of cyclin (PCNA) during S‐phase are not triggered by post‐translational modifications that are expected to moderately affect its chargeFEBS Letters, 1985
- Identity of the proliferating cell nuclear antigen and cyclinNature, 1984
- Cyclin: A nuclear protein whose level correlates directly with the proliferative state of normal as well as transformed cellsLeukemia Research, 1984
- Identification of a nuclear and of a cytoplasmic polypeptide whose relative proportions are sensitive to changes in the rate of cell proliferationExperimental Cell Research, 1981
- A nuclear antigen associated with cell proliferation and blast transformation.The Journal of Experimental Medicine, 1981
- Coexistence of three major isoactins in a single sarcoma 180 cellCell, 1981
- A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells.The Journal of cell biology, 1980