Transcriptional Mapping of the Coat Protein Gene of Tomato Golden Mosaic Virus

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Abstract
Polyadenylated RNA was isolated from Nicotiana benthamiana plants infected with tomato golden mosaic virus (TGMV). Northern hybridization with a strand-specific probe of cloned TGMV DNA A revealed a 0.9kb transcript with the same orientation as virion DNA. The positions of the 5'' and 3'' termini of the transcript, which were mapped by nuclease protection and primer extension techniques, innicated that it corresponded to the virus coat protein mRNA and enabled the likely functional promoter and polyadenylation signals to be identified.