Spontaneous Dissociation of Human Pituitary Luteinizing Hormone in Solution*

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Abstract
Aliquots of a solution of highly purified human pituitary LH [luteinizing hormone] (hLH) were incubated with variations in temperature and time. The incubates were chromatographed on Sephadex G-100 as well as on DEAE-Sephadex A25. The column effluents were assayed in radioligand assay and in specific hLH.alpha.- and hLH.beta.-RIA systems. There is spontaneous dissociation of hLH at elevated temperatures under otherwise normal conditions concerning, for example, pH and ionic strength. The degree of dissociation is both time and temperature dependent. This dissociation is not due to proteolytic enzymes. One should be alert when using hLH subunit RIA [radioimmunoassay] systems at elevated temperatures for measuring hLH levels, e.g., in clinical samples, because artificial high levels may be obtained.

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