The Synthesis of Virus-Specific Single-Stranded RNA in Larvae of Bombyx mori Infected with a Cytoplasmic Polyhedrosis Virus

Publisher Website
Google Scholar
Abstract
RNA synthesis in larvae of Bombyx mori was examined by theincorporation of 32P into purified single- and synthesis double-stranded RNAs. In larvae infected with a cytoplasmic polyhedrosis virus (CPV), both single- and double-stranded RNA were synthesized after pre-treatment with 10 µg actinomycin D. Approximately 70 % of the single-stranded RNA synthesized in these larvae re-annealed with heat-denatured RNA extracted from purified virions of B. mori CPV. It did not self-anneal, nor did it anneal significantly with RNA from Arctia caja CPV, or from B. morilaτvae. This suggested that the single-stranded RNA is largely virus-specific and represents viral messenger RNA.

This publication has 0 references indexed in Scilit: