Porcine Hepatocytes From Slaughterhouse Organs

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Abstract
Most recent strategies for the development of hybrid artificial liver devices focus on the use of parenchymal liver cells (hepatocytes). For clinical application of these devices, a sufficient cell supply is mandatory. Because human liver tissue is rarely available, isolated porcine hepatocytes from laboratory animals have been suggested for use in bioartificial livers. The authors introduce a modified isolation protocol to yield large scale numbers of viable porcine hepatocytes from slaughterhouse organs. Perfusion and enzymatic digestion of the left medial liver lobe (n = 74) resulted in 1.0 ± 0.3 × 107 viable hepatocytes per gram of tissue, and an overall yield of 1.92 ± 0.5 × 109 viable cells per isolation (viability: 93 ± 2%). Collagen gel immobilization maintained morphologic integrity and functional activity of hepatocyte cultures over long-term periods. Cell morphology, as assessed by light microscopic evaluation, was maintained for 2 weeks. Stable DNA content (51 ± 5μg) and low values of alanine aminotransferase release (8μU/hr/μg DNA) indicated structural stability of cultures after a short period of post isolational adaptation. Albumin secretion (4.5μg/hr/μg DNA) and persistent Cytochrome P450 IA1 dependent deethylation of 7-ethoxycoumarin (4.5 nmol/hr/μg DNA) indicated long-term metabolic activity of cultured hepatocytes. Hepatocytes from livers of slaughtered pigs represent an unlimited resource of viable material for cell culture, and their usefulness as functional units of bioartificial liver support devices should be tested.ASAIO Journal1995; 41:189-193.

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