A Method for Quantitative Amino Acid Analysis Using Precolumn o-Phthalaldehyde Derivatization and High Performance Liquid Chromatography

1 May 1981

journal article
Published by Oxford University Press (OUP) in Journal of Chromatographic Science

Vol. 19 (5) , 259-265
https://doi.org/10.1093/chromsci/19.5.259

Abstract

A fluorometric procedure for quantitative amino acid analysis of a typical peptide hydrolysate is presented. The amino acids were derivative with o-phthalaldehyde (OPA) prior to injection into the high performance liquid chromatographic (HPLC) apparatus. The OPA-amino acid adducts were resolved using a linear gradient of acetonitrile against an initial solvent consisting of 0.01875 M triethylamine acetate (TEA-A), pH 7.5. Complete analysis required 46 min, with an injection-to-injection time of 56 min. The lower limit of quantitation using the equipment specified was found to be 100 pmoies, with a lower detection limit of 25 pmoles.

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