Biosensor assay of sulfadiazine and sulfamethazine residues in pork
- 1 September 2000
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 125 (10) , 1771-1774
- https://doi.org/10.1039/b004835f
Abstract
Biosensor-based immunochemical screening assays for the detection of sulfadiazine (SDZ) and sulfamethazine (SMT) in muscle extract from pigs were developed. Samples were extracted with aqueous buffer and then centrifuged. This simple and straightforward preparation allowed up to 40 samples to be processed and analysed in 1 d. The limits of detection for the assays were found to be 5.6 ng g−1 for SDZ and 7.4 ng g−1 for SMT. These figures were well below the European and US legal limits for sulfonamides (100 ng g−1). The precision (RSD) between runs was N4-acetyl metabolite while the HPLC method used for confirmatory analysis detected only the parent sulfonamide. This gave rise to some false positive results and highlighted the need to use real samples in evaluating any assay thoroughly. False negative results were not obtained.Keywords
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