Mutational Analysis of the TolA C-Terminal Domain ofEscherichia coliand Genetic Evidence for an Interaction between TolA and TolB

Abstract
The Tol proteins are involved in the outer membrane stability of gram-negative bacteria. The C-terminal domain of TolA was mutagenized to identify residues important for its functions. The isolation of suppressor mutants of tolA mutations in the tolB gene confirmed an interaction between TolAIII and the N-terminal domain of TolB.