Application of Gersh's Ferrocyanide Technique to the Study of Experimental Renal Disease

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Abstract
Gersh showed, using the freezing-drying technic, that Na ferrocyanide was excreted solely by glomerular filtration without tubular resorption or secretion. The original method has been slightly modified and used in the study of renal function in exptl. kidney lesions in dogs and rabbits. After intraven. injns. of Na ferrocyanide, kidney sections are prepared by the freezing-drying method and exposed to ferric chloride precipitating ferric ferrocyanide in the sites where the ferrocyanide had been present. In normal kidneys, deposits of ferric ferrocyanide can be demonstrated within Bowman''s space and in the lumen of all the tubules. In acute unilateral hydronephrosis the precipitate was plentiful within the glomerular spaces and proximal convoluted tubules while scanty or absent in the more distal tubules; it was abundant throughout the tubular system of the control kidney. In acute HgCl2 poisoning the ferrocyanide deposit was plentiful in the glomerular spaces; the necrotic proximal convoluted tubule cells were stained diffusely blue, but no precipitate was found in the rest of the tubular system.

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