Identification of the minor component of a mixed stain by using mismatch primer-induced restriction sites in amplified mtDNA
- 9 April 2003
- journal article
- case report
- Published by Springer Nature in International journal of legal medicine
- Vol. 117 (3) , 160-164
- https://doi.org/10.1007/s00414-002-0342-7
Abstract
We report a case in which STR typing failed to identify the minor component of a mixed saliva stain, but a mitochondrial restriction analysis succeeded in discriminating between the two components. To identify the nt16093 and nt16265 transitions, the template was amplified with the mismatch primers L16092-mm16085 and H16266-mm16269. In the presence of the transitions the mismatch primers created a BsaB I and a Cac8 I restriction site, respectively. Subsequently, aliquots were restricted separately using the enzymes Cac8 I and BsaB I which clearly identified the minor stain component.Keywords
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