Monosialogangliosides of Rabbit Skeletal Muscle. Characterization of N-Acetylneuraminosyl Lacto-N-noroctaosyl Ceramide1

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Abstract
Rabbit skeletal muscle contained 28.4 nanomol/g wet weight of lipid-bound sialic acid, and 73.4% of the total lipid-bound sialic acid was recovered in a monosialo-ganglioside fraction. Monosialoganglioside components were isolated and the structures were determined by exoglycosidase treatment and permethylation anal-ysis. The major monosialoganglioside was GM3 (73.2% of monosialogangliosides), and gangliosides of the lacto-series with at least three repeating units of lactosamine, Gal(β, 1–4)GlcNAc(β, 1–3), were identified. A newly found ganglioside was N-acetylneuraminosyl lacto-N-noroctaosyl ceramide, NeuAc(α, 2–3)Gal(β, 1–4)GlcNAc (β, 1–3)Gal(β, 1–4)G1cNAc(β, 1–3)Gal(β, 1–4)GlcNAc(β, 1–3)Gal(β, 1–4)Glc(β, 1–1)cer-amide. The amounts of lacto-series gangliosides in the monosialoganglioside fraction were as follows: sialosyl lacto-N-neotetraosyl ceramide (9.8%), sialosyl lacto-N-norhexaosyl ceramide (11.9%) and sialosyl lacto-N-noroctaosyl ceramide (5.1%). N-Glycolylneuraminic acid was found in sialosyl lacto-N-neotetraosyl ceramide and sialosyl lacto-N-norhexaosyl ceramide but the amount was less than 0.2%. The mobilities of lacto-series gangliosides were compared with those of ganglio-series gangliosides. Fatty acid and long chain base compositions were also delermined.

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