Retardation of Growth Rate in Aedes Aegypti (L.) Larvae Exposed to Vital Dyes1

Abstract
Exposure to either neutral red, nile blue A or methylene blue extended the developmental period of A. aegypti (L.) larvae. The rate of pupation decreased and the mortality increased as a function of increasing concentration. Pupal weights were adversely affected in females but not in males. These adverse effects caused by commonly used “vital” dyes demonstrate the need for increased caution in their use.

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