CHYMOTRYPSINOGEN: INCREASED RESOLUTION AND ABSOLUTE CONFIGURATION

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Abstract
A new set of Fourier maps have been calculated for type F chymotrypsinogen from phases obtained by mul-tiple isomorphous replacement, including terms out to a minimum Bragg spacing of 4 A. Features can now be seen which seem to be in-dividual curved backbone chains with side-chain bulges. There is nothing which would correspond to an [alpha]-helix segment of more than a single turn. Disulfide bridges are still not obvious, and it is still not possible to follow a unique path for the backbone chain. The absolute configuration of the chymotrypsinogen molecule has been established with the aid of anomalous scattering data from the Hg14[long dash]derivative. The correct configuration is the mirror image of our previously pub-lished model. It appears unlikely that our present heavy-atom derivatives would enable the resolution to be extended much further. Possible approaches to the complete structure are discussed.

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