Activation of phospholipase D in rabbit neutrophils by fMet‐Leu‐Phe is mediated by a pertussis toxin‐sensitive GTP‐binding protein that may be distinct from a phospholipase C‐regulating protein

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Abstract
Stimulation by N‐formyl‐Met‐Leu‐Phe (fMLP) of rabbit peritoneal neutrophils, in which phosphatidylcholine was preferentially labeled with l‐O‐[3H]octadecyl lyso platelet‐activating factor, activated phospholipase D, resulting in the formation of [3H]PA from [3H]PC. A direct activator of GTP‐binding proteins (G‐proteins), NaF, also stimulated [3H]PA formation. fMLP‐stimulated [3H]PA formation was inhibited by pertussis toxin (IAP) in a time‐ and dose‐dependent manner. IAP also inhibited fMLP‐stimulated IP3 formation, but the inhibition of IP3 formation was significantly greater than that of [3H]PA formation. These results indicate that activation of phospholipase D by fMLP in rabbit neutrophils is mediated by an IAP‐sensitive G‐protein that may be distinct from a phospholipase C‐regulating protein.

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