THE ASSAY OF ESTROGENS IN THE CHICK BY ORAL ADMINISTRATION1

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Abstract
THIS communication is concerned with the bioassay of estrogens in chicks when the material is administered orally and with the relative activities of various estrogens by the oral route. animals, materials, and methods Various preparations of estrogen sulfates were supplied by Endo Products Inc.; the stilbestrol was supplied by Winthrop-Stearns Inc.; and methoxy bisdehydrodoisynolic acid (MDDA) and estradiol dipropionate was supplied by Ciba Pharmaceutical Products Inc. The Premarin (Ayerst, McKenna and Harrison) was purchased on the open market. Estynyl estradiol was supplied by the Schering Corporation. The experimental design of Bliss (1944) was employed for statistical calculations of potency ratio. This consisted essentially in setting up four groups of animals, two groups on the standard and two groups on the unknown. The concentrations of the high and low doses were determined by the following relationship: Using this experimental design, the combined slope (b), the significance of the differences of the slopes of unknown and standard

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