Application of physico-chemical typing methods for the epidemiological analysis ofSalmonella enteritidisstrains of phage type 25/17
- 1 December 1994
- journal article
- research article
- Published by Cambridge University Press (CUP) in Epidemiology and Infection
- Vol. 113 (3) , 411-424
- https://doi.org/10.1017/s0950268800068424
Abstract
SUMMARY: Eighty-nineSalmonella enteritidisphage type 25/17 strains isolated from a localized outbreak in the German state Nordrhein-Westfalen (outbreak NWI) could not be further differentiated by biochemotyping and plasmid pattern analysis. They were submitted to a complex typing system consisting of modern physico-chemical analytical procedures. In lipopolysaccharide pattern analysis the strains proved to be homogeneous. In multilocus enzyme electrophoresis, outer membrane and whole cell protein pattern (WCPP) analysis, and Fourier transform infrared (FT–IR) spectroscopy (increasing extent of differentiation in the given order) strains deviating from each basal pattern were found. The extent of correspondence in these deviations was satisfactory.Forty-six strains of the same sero- and phage type, however, obtained from different outbreaks, were additionally typed. The results obtained with them indicate that the data of the first group were not restricted to strains from outbreak NWI, but of general validity.It was found that both WCPP and FT–IR represent valuable methods for the sub-grouping of bacteria.Keywords
This publication has 15 references indexed in Scilit:
- Comparison of multilocus enzyme electrophoresis (MEE), ribotyping, restriction enzyme analysis (REA) and phage typing for typing ofListeria monocytogenesEpidemiology and Infection, 1993
- Repeat unit polysaccharides of bacteria: a model for polymerization resembling that of ribosomes and fatty acid synthetase, with a novel mechanism for determining chain lengthMolecular Microbiology, 1993
- Biochemical and molecular characterization ofSalmonella entericaserovarberta, and comparison of methods for typingEpidemiology and Infection, 1992
- A comparison between low background silver diammine and silver nitrate protein stainsElectrophoresis, 1992
- Classification and identification of bacteria by Fourier-transform infrared spectroscopyMicrobiology, 1991
- A New and Rapid Method for the Assay of Bacterial Fatty Acids Using High Resolution Capillary Gas Chromatography and Trimethylsulfonium HydroxideZentralblatt für Bakteriologie, 1990
- A phage-typing scheme for Salmonella enteritidisEpidemiology and Infection, 1987
- Numerical analysis of electrophoretic protein patterns ofProvidencia rustigianiistrains from human diarrhoea and other sourcesJournal of Applied Bacteriology, 1987
- Summary of a Workshop on the Clone Concept in the Epidemiology, Taxonomy, and Evolution of the Enterobacteriaceae and other BacteriaThe Journal of Infectious Diseases, 1983
- Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bandsFEBS Letters, 1975