Delayed-Type Hypersensitivity Measured as an Increase of Ear Thickness in Guinea Pigs

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Abstract
Determination of swelling at an intracutaneous test site in the pinna of the ear of guinea pigs immunized with protein antigens in complete Freund’s adjuvant was found to be a more sensitive assay of delayed-type hypersensitivity than the measurement of flank skin erythema and induration. In fact, 100 times less specific antigen was needed to detect 24-hour reactivity in the pinna of the ear. Reactivity early after sensitization (cutaneous basophil hypersensitivity), however, was best revealed as an erythematous lesion of the flank skin 24 h after testing.