THE ESTIMATION OF TRYPSIN

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Abstract
Details are given of a colorimetric method for the estimation of trypsin that can be utilized for the evaluation of the proteolytic activity of pancreatic secretion. Being an adaptation of a method that has been used successfully in a clinical laboratory for many years, it is suitable for routine clinical use. Like Anson's method it depends upon the colour reaction between the tyrosine–tryptophane radicals of a protein substrate and Folin's phenol reagent, but embodies the following features: the results cannot be influenced by the presence of peptidases in the enzyme solution, since the procedure is based upon the estimation of a casein substrate before and after digestion, and not upon the digestion products; the digestion is carried out at 37 °C.; by reference to a curve the enzyme content can be expressed in terms of trypsin nitrogen. Results obtained with this method showed a reasonable degree of constancy, the average deviation from the mean being ±3.3% and the maximum deviation, ±8.4%.

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