FREQUENCY, SPECIFICITY, AND PHENOTYPE OF CLONALLY GROWING HUMAN ALLOREACTIVE INTERLEUKIN-2—SECRETING HELPER T LYMPHOCYTE PRECURSORS
- 1 November 1990
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 50 (5) , 850-855
- https://doi.org/10.1097/00007890-199011000-00021
Abstract
Limiting dilution cultures were performed to detect allospecific IL-2-secreting helper T lymphocyte precursors (HTL-p) among human peripheral blood mononuclear cells, E-rosette-purified (E+) and cell-sorter-separated CD4+/8- as well as CD4-/8+ T cell subsets. Splitwell cultures were set up prior to restimulation to assess the antigen specificity of the response. Frequencies of alloreactive IL-2-secreting HTL-p in fully HLA-mismatched responder/stimulator cell combinations ranged from 1/200 to 1/900 (among PBMNC), from 1/50 to 1/301 (among E+ T cells), from 1/36 to 1/220 (among CD4+ T cells), and from 1/38 to 1/450 (among CD8+ T cells). Allospecificity of effector T cells was demonstrated by a strong decline of frequencies obtained after restimulation against unrelated third-party antigens. In clonal segregation analysis, the vast majority of IL-2-secreting progeny (80-90%) were exclusively specific for the original stimulating alloantigen. Finally, the allele specificity of human alloreactive HTL-p was revealed by comparing frequency estimates obtained after restimulation with partially identical stimulator/third-party antigen combinations.This publication has 23 references indexed in Scilit:
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