Dual regulation of the T‐type Ca2+ current by serum albumin and β‐estradiol in mammalian spermatogenic cells
- 21 June 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 475 (3) , 251-256
- https://doi.org/10.1016/s0014-5793(00)01688-4
Abstract
This study provides evidence for a novel mechanism of voltage-gated Ca2+ channel regulation in mammalian spermatogenic cells by two agents that affect sperm capacitation and the acrosome reaction (AR). Patch-clamp experiments demonstrated that serum albumin induced an increase in Ca2+ T current density in a concentration-dependent manner, and significant shifts in the voltage dependence of both steady-state activation and inactivation of the channels. These actions were not related to the ability of albumin to remove cholesterol from the membrane. In contrast, β-estradiol significantly inhibited Ca2+ channel activity in a concentration-dependent and essentially voltage-independent fashion. In mature sperm this dual regulation may influence capacitation and/or the AR.Keywords
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