The Acceptor Availability at Photosystem I and ABA Control Nuclear Expression of 2-Cys Peroxiredoxin-A in Arabidopsis thaliana
Open Access
- 15 August 2004
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 45 (8) , 997-1006
- https://doi.org/10.1093/pcp/pch114
Abstract
The redox-regulated 2-Cys peroxiredoxin-A (2CPA) promoter, which drives expression of a dominant chloroplast antioxidant enzyme, responds to signals originating from the photosynthetic electron transport downstream of PSI. Modulation of CO2- and NO3– -reduction rates in reporter gene plants expressing glucuronidase under control of the Arabidopsis thaliana 2CPA promoter revealed that promoter activity correlates with the availability of electron acceptors at PSI. The photosynthetic redox-regulation can be simulated by oxidant and antioxidant treatments. Inhibitor studies with PD98059 and staurosporine showed that a mitogen-activated protein kinase kinase transmits the oxidative response, while the antioxidant signal is transmitted by a serine/threonine kinase. Analysis of 2CPA promoter regulation in the abscisic acid (ABA)-biosynthetic mutants aba2 and aba3 and the ABA-insensitive mutants abi1 and abi2 support a regulatory circuitry in which the redox signal cross-talks with the ABA-signaling cascade downstream of ABI1 and ABI2.Keywords
This publication has 54 references indexed in Scilit:
- Characterization of Mutants in Arabidopsis Showing Increased Sugar-Specific Gene Expression, Growth, and Developmental ResponsesPlant Physiology, 2004
- Regulation and Role of the ArabidopsisAbscisic Acid-Insensitive 5Gene in Abscisic Acid, Sugar, and Stress ResponsePlant Physiology, 2002
- A critical role for ethylene in hydrogen peroxide release during programmed cell death in tomato suspension cellsPlanta, 2002
- Antisense Suppression of 2-Cysteine Peroxiredoxin in Arabidopsis Specifically Enhances the Activities and Expression of Enzymes Associated with Ascorbate Metabolism But Not Glutathione MetabolismPlant Physiology, 2000
- ABA activation of an MBP kinase in Pisum sativum epidermal peels correlates with stomatal responses to ABAJournal of Experimental Botany, 2000
- Protective Function of Chloroplast 2-Cysteine Peroxiredoxin in Photosynthesis. Evidence from Transgenic Arabidopsis1Plant Physiology, 1999
- Transient Gene Expression in Protoplasts of Arabidopsis thalianaPublished by Springer Nature ,1998
- The plant 2‐Cys peroxiredoxin BAS1 is a nuclear‐encoded chloroplast protein: its expressional regulation, phylogenetic origin, and implications for its specific physiological function in plantsThe Plant Journal, 1997
- Sensitivity to abscisic acid of guard-cell K+ channels is suppressed by abi1-1, a mutant Arabidopsis gene encoding a putative protein phosphatase.Proceedings of the National Academy of Sciences, 1995
- Transient Expression Activity of RbcS Promoter Regions from Brassica napus in Mesophyll Protoplasts from Nicotiana tabacum*Botanica Acta, 1995