Analysis of the Glycoproteins of Murine Tumour Cell Lines with 125I-Concanavalin A in Two-Dimensional Electrophoresis Gels

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Abstract
The combination of 2-dimensional gel electrophoresis and post-electrophoretic staining with 125I-labeled concanavalin A was used to compare the glycoproteins of murine tumor cell lines. Comparison between different cell lines showed that there were about 8 common glycoproteins. The rest of the glycoproteins were generally unique to particular cells. Thus the P815 [mouse mastocytoma] cell could be distinguished from 13 other murine cell lines by its glycoprotein pattern. The specific glycoproteins of each cell line were unaffected by culture in vivo, virus infection or hybridization. Different clones from the same cell line gave identical patterns. Crude membrane preparations and glycoproteins purified from cell lysates by affinity chromatography on concanavalin/agarose gave the same patterns as whole cells. Thus the glycoproteins of murine tumor cells appear to be a stable characteristic which can provide specific markers for the identification of tumor cell lines.