Improvements in the Permanent Root Tip Squash Technic

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Abstract
Root-tip squashed prepns. mounted in the acetic acid-corn syrup mounting medium (Hillary, Stain Technol. 13: 161-167. 1938) sometimes become decolorized and worthless, though the corn syrup-water mounting medium has always given good results. Because of the variability of commercial corn syrups the author recommends dioxan-balsam mounts, made as follows: Fix 30-60 min., preferably in a Navashin type fixative; wash 30 min. or longer in running water; treat 15 min. in 4% (by volume) NH40H at 60[degree] C in a corked vial; wash 30 min. in running water to remove soluble pectate; hydrolyze 8 min. in N HCl at 60[degree] C; stain 20 min. or longer in fuchsin-sulfurous-acid; bleach in 3 changes of SO2-water for at least 10 min. per change; dehydrate in 3 changes of dioxan of 3 min. each; place a root tip in a drop of dioxan-balsam on a slide, separate into fine longitudinal strands by means of sharp needles; press on a cover slip.

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