Properties of Two Clostridia Strains Acting as Catalysts for the Preparative Stereospecifïc Hydrogenation of 2-Enoic Acids and 2-Alken-1-ols with Hydrogen Gas

Publisher Website
Google Scholar
Abstract
A Clostridium strain growing on crotonate/hydrogen carbonate, which is able to hydrogenate stereospecifically 2-enoates and unsaturated compounds with H2, was isolated and methods for its propagation elucidated. For this strain and for C. kluyveri DSM 555 grown on ethanol/acetate/hydrogen carbonate, samples of 200 l batches were assayed to determine the hydrogenation activity for (E)-2-methyl-2-butenoate and (E)-2-buten-1-ol as a function of time during the exponential and stationary growth phases. For the strain growing on crotonate/hydrogen carbonate, the hydrogenation rate as a function of substrate concentration, pH and temperature was measured. Storage conditions for both strains are given.