AN INTRACELLULAR HEMOLYSIN OF GROUP A STREPTOCOCCI

Publisher Website
Google Scholar
Abstract
A hemolysin can be obtained from sonic disrupted group A streptococcal cells which is apparently distinct from streptolysins O and S. The washed cells from a 16-hour culture are suspended in isotonic PO4 buffer pH 6.5 and subjected to sonic vibration for periods up to 8 hours. The hemolysin is very labile and a low temperature must be maintained throughout. Extended treatment of the streptococci in the sonic oscillator increases the amount of hemolysin released from the cells. The hemolytic activity of the sonic extract can be increased by exposure of the extract to a pH of 2 for 1 to 5 minutes followed by neutralization.

This publication has 6 references indexed in Scilit: