Cellobiose Uptake in the Hyperthermophilic Archaeon Pyrococcus furiosus Is Mediated by an Inducible, High-Affinity ABC Transporter
Open Access
- 1 September 2001
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (17) , 4979-4984
- https://doi.org/10.1128/jb.183.17.4979-4984.2001
Abstract
The hyperthermophilic archaeon Pyrococcus furiosus can utilize different β-glucosides, like cellobiose and laminarin. Cellobiose uptake occurs with high affinity ( K m = 175 nM) and involves an inducible binding protein-dependent transport system. The cellobiose binding protein (CbtA) was purified from P. furiosus membranes to homogeneity as a 70-kDa glycoprotein. CbtA not only binds cellobiose but also cellotriose, cellotetraose, cellopentaose, laminaribiose, laminaritriose, and sophorose. The cbtA gene was cloned and functionally expressed in Escherichia coli. cbtA belongs to a gene cluster that encodes a transporter that belongs to the Opp family of ABC transporters.Keywords
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