Cell Separation on Affinity Columns: The Preparation of Pure Populations of Anti-Hapten Specific Lymphocytes

Open Access
Publisher Website
Google Scholar
Abstract
Procedures were developed for purifying anti-hapten specific lymphocytes on an immunoadsorbent. Mouse spleen cells were filtered through large polyacrylamide beads with covalently attached β-lactoside hapten groups. Specifically binding cells were eluted with hapten from washed affinity beads. Hapten-eluted spleen cell populations derived from non-immune mice were much smaller in number than those from mice immunized against hemocyanin azophenyl-β-lactoside. Pure populations of anti-hapten specific cells from immune spleens were greatly enriched in indirect plaque-forming cells against β-lactoside-coupled sheep red blood cells.