Isolation and study of rabbit antibodies specific for certain of the antigenic groups of human serum albumin

Publisher Website
Google Scholar
Abstract
An insoluble adsorbent specific for certain anti-(human serum albumin) antibodies was prepared by coupling to p-aminobenzylcellulose a fragment of the human-serum-albumin molecule called "inhibitor." Rabbit anti-(human serum albumin) antibodies reacting with "inhibitor" were specifically adsorbed on the "inhibitor" -benzylcellulose conjugates and were desorbed by glycine-hydrochloric acid buffer, pH 1.0. The isolated antibodies did not give precipitates with human serum albumin or "inhibitor" but did agglutinate red cells sensitized with human serum albumin or "inhibitor". They formed with human serum albumin or "inhibitor" soluble complexes which have been studied by ultra-centrifugal analysis. These antibodies were bivalent and reacted with at least, and probably only, two sites on the human-serum-albumin or "inhibitor" molecules.