ALTERATION OF THE CIRCULATING LIFE AND ANTIGENIC PROPERTIES OF BOVINE ADENOSINE-DEAMINASE IN MICE BY ATTACHMENT OF POLYETHYLENE-GLYCOL
- 1 January 1981
- journal article
- research article
- Vol. 46 (3) , 649-652
Abstract
Polyethylene glycol was attached covalently to adenosine deaminase (ADA) using cyanuric chloride as the coupling agent. The modified adenosine deaminase (PEG-ADA) appears to lose its immunogenicity in mice following multiple i.v. injections. PEG-ADA does not react with antibodies raised against native ADA. The circulating half-life (T1/2) of PEG-ADA was increased to 28 h. The lack of detectable antibody formation and long circulating life may make PEG-ADA suitable for treating human ADA deficiency.This publication has 5 references indexed in Scilit:
- Alteration of immunological properties of bovine serum albumin by covalent attachment of polyethylene glycol.Journal of Biological Chemistry, 1977
- Enzyme Replacement Therapy for Adenosine Deaminase Deficiency and Severe Combined ImmunodeficiencyNew England Journal of Medicine, 1976
- RECONSTITUTION OF B AND T LYMPHOCYTE FUNCTION IN SEVERE COMBINED IMMUNODEFICIENCY DISEASE AFTER TRANSPLANTATION WITH THYMIC EPITHELIUMThe Lancet, 1976
- Combined immunodeficiency disease associated with adenosine deaminase deficiency: Report on a Workshop Held in Albany, New York, October 1, 1973The Journal of Pediatrics, 1975
- Determination of free amino groups in proteins by trinitrobenzenesulfonic acidAnalytical Biochemistry, 1966