Monoclonal antibodies to a benzo[a]pyrene diolepoxide modified protein
- 1 January 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 7 (3) , 441-444
- https://doi.org/10.1093/carcin/7.3.441
Abstract
Monoclonal antibodies have been developed which specifically recognize protein modified by 7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE-I). These antibodies have been characterized as to sensitivity and specificity by an enzyme-linked immunosorbent assay. They react with BPDE-I-modified proteins and do not cross-react with non-modified protein. All the antibodies have higher sensitivity toward BPDE-I-tetraols than to BPDE-I-modified protein. They cross-react with BPDE-I-deoxyguanosine, BPDE-I-DNA and BPDE-II-rabbit serum albumin. Because of the high antibody sensitivity for tetraols, an immunoassay was developed for quantitating DNA and protein adducts based on release of tetraols from modified samples. This approach was validated using radiolabeled BPDE-I modified DNA and protein.This publication has 6 references indexed in Scilit:
- Ubiquitous binding of benzo[a]pyrene metabolites to DNA and protein in tissues of the mouse and rabbitChemico-Biological Interactions, 1984
- Binding of chemical carcinogens and mutagens to rat hemoglobinChemico-Biological Interactions, 1980
- QUANTITATION OF BENZO(A)PYRENE-DEOXYGUANOSINE ADDUCTS BY RADIOIMMUNOASSAY1980
- Evaluation of genetic risks of alkylating agents IV. Quantitative determination of alkylated amino acids in haemoglobin as a measure of the dose after treatment of mice with methyl methanesulfonateMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
- Alkylation of DNA and proteins in mice exposed to vinyl chlorideBiochemical and Biophysical Research Communications, 1977
- Nucleoside adducts from the in vitro reaction of benzo[a]pyrene-7,8-dihydrodiol 9,10-oxide or benzo[a]pyrene 4,5-oxide with nucleic acidsBiochemistry, 1977