STUDY OF SURFACE SECRETION OF BRONCHIOLE USING AUTORADIOGRAPHY

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Abstract
The chemical nature and cell of origin of the surface secretion of the bronchiole was studied using radioautography after the injection of labeled leucine, glucosamine and galactose. Rat lungs were perfused with mammalian Ringer''s solution with added albumin. A 10-min pulse of L-leucine labeled with 3H was administered, followed by perfusion with nonradioactive solution for 0.25-4 h. Similar studies were performed with [3H] glucosamine and [3H] galactose. Heavy labeling of the Clara cells of bronchioles was obtained after injection of [3H] L-leucine. Labeling of alveolar wall cells was also obtained. Labeling of the surface of the bronchiole was much heavier than the labeling of the surface of the alveoli. No specific labeling of bronchiolar cells was obtained with [3H] glucosamine and [3H] galactose. The results provide added evidence for the protein nature of the surface layer of the bronchiole and for the Clara cell as its cell of origin.

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