Ultrastructural Localization of Prostaglandin Endoperoxide Synthase (Cyclooxygenase) to Isolated, Purified Fractions of Guinea Pig Peritoneal Macrophage and Line 10 Hepatocarcinoma Cell Lipid Bodies
- 1 January 1993
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 101 (2) , 136-142
- https://doi.org/10.1159/000236511
Abstract
Subcellular fractions of purified cytoplasmic, nonmembrane-bound lipid bodies were prepared from [3H]-arachidonic acid-labeled guinea pig peritoneal macrophages and line 10 hepatocarcinoma cells. These fractions, which contained [3H]-arachidonyl lipids, were shown to be devoid of contaminating cellular membranes by electron microscopy, and to contain prostaglandin endoperoxide (PGH) synthase by postembedding immunogold electron microscopy. These findings support a proposed role for these lipid-rich organelles in the generation of eicosanoids by oxidative metabolism of arachidonate in the cyclooxygenase pathway of inflammatory and neoplastic cells.Keywords
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