Transmission of chloroplast genes in crosses between Chlamydomonas reinhardtii diploids: Correlation with chloroplast nucleoid behavior in young zygotes
- 1 April 1984
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 8 (3) , 223-229
- https://doi.org/10.1007/bf00417820
Abstract
In crosses between diploid strains of Chlamydomonas reinhardtii, the mode of chloroplast gene transmission was studied and correlated with the behavior of DAPI-stained chloroplast nucleoids in young tetraploid zygotes. Phenotypically plus (female) diploids with mating-type genotypes of mt +/mt + or mt +/0 (hemizygote) and phenotypically minus (male) diploids with genotypes of mt +/mt − or mt −/mt − or 0/mt − were obtained by either crosses or polyethylene glycol induced somatic fusions between haploid strains. When crosses were made between mt +/mt + or mt +/0 and mt −/mt − or 0/mt −, the transmission of chloroplast genes occurred mostly from the female parent, indicating a typical maternal inheritance. In these cases the chloroplast nucleoids of one of the two parents disappeared within 2.5 h after mating. In contrast, when female diploids were crossed to mt +/mt − male diploids, the chloroplast genes from both parents were transmitted to the progeny at equal frequency. The chloroplast nucleoids derived from the two parents were conserved for at least 2.5 h after mating. It is concluded that the chloroplast genes derived from the heterozygous male (mt +/mt −) are protected from the degradation that takes place soon after zygote formation, by virtue of the presence of mt + gene although this allele is recessive with respect to the phenotypic expression of sexuality in diploids.This publication has 39 references indexed in Scilit:
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