Simultaneous Decrease in Deamination of 5′‐Adenylic Acid and 5′‐Deoxy‐5′‐S‐isobutylthioadenosine in Chick‐Embryo Fibroblasts Infected by Rous Sarcoma Virus

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Abstract
The rate of deamination of 5''-deoxy-5''-S-isobutylthioadenosine [(iBuS)5'' Ado] in chick embryo fibroblasts was substantially reduced after their infection and morphological transformation by Rous sarcoma virus. Concomitant with the reduction in rate of (iBuS)5'' Ado deamination, there was a decrease in adenosine deaminase and 5''-adenylic acid deaminase activities. The drop of these activities was related to infection, and not to the expression of the src gene. (iBuS)5'' Ado was deaminated by at least 3 enzymes or isoenzymes whose apparent MW were estimated to be 295,000, 121,000 and 37,000, respectively. Two of these enzymes have been characterized as 5''-adenylic acid deaminase and the heavy form of adenosine deaminase, respectively. [(iBuS)5''-Ado, a synthetic analog of S-adenosyl-L-homocysteine, inhibits cell transformation of chick embryo fibroblast infected by Rous sarcoma virus and replication of oncogenic RNA and DNA viruses.].