Lactate Dehydrogenase Isoenzyme Patterns in Oocytes, Unfertilized Eggs and Embryos from Mice and Cattle

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Abstract
Lactate dehydrogenase isoenzyme patterns in maternal tissues, oocytes, unfertilized eggs and embryos from mice and cattle were examined by polyacrylamide micro disc-gel electrophoresis. The results confirm that with respect to LDH content two types of oocytes, unfertilized eggs, and embryos contained only LDH-1 (β subunits), while similar material from cattle exhibited LDH-1 and LDH-2 indicating the presence of both α and β subunits. Maternal tissues such as heart, muscle and kidney, contained a greater number of lactate dehydrogenase isoenzymes than denuded oocytes, unfertilized eggs and embryos within species. Further, the intensity of the isoenzyme bands following staining increased toward LDH-4 in maternal tissues of cattle, while in denuded oocytes, unfertilized eggs and embryos of both the mouse and the cow the intensity increased toward LDH-1. These findings suggest that LDH activity found in oocytes, unfertilized eggs and embryos of mice and cattle is a result of de novo synthesis during oogenesis and/or the preimplantation period of development and not a result of contamination or uptake from maternal sources. Copyright © 1976. American Society of Animal Science . Copyright 1976 by American Society of Animal Science

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