REQUIREMENT FOR NON T-CELLS IN GENERATION OF CYTOTOXIC T LYMPHOCYTES (CTL) INVITRO .2. CHARACTERIZATION OF ACTIVE-CELLS IN SPLEEN OF NUDE MICE

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 118  (1) , 166-174
Abstract
Small numbers of LN [lymph node] cells will produce many more cytotoxic lymphocytes on in vitro culture with allogeneic stimulator cells if spleen cells from nu/nu mice are also present throughout the culture period. All cytotoxic cells produced are T [thymus-derived] cells and arise from precursors in the LN component. The nude spleen component appears to be providing a required non-T cell which was lost from the LN component through dilution. At least 2 active subpopulations of cells, differing in sedimentation velocity, adherence properties, radiation sensitivity and antigen recognition properties can be identified in the nu/nu spleen. The first, the dominant activity in normal nu/nu spleen, is nonadherent, radiation sensitive, and can synergize with syngeneic or allogeneic LN cells provided both are different from the same alloantigens in the stimulator population. The 2nd, found in nu/nu spleen cells precultured with alloantigen, sediments more rapidly, is adherent and radiation resistant, and need not be allogeneic to the stimulator cells to synergize with LN cells. The 1st subpopulation may give rise to the 2nd.

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