THE MINUTE-VOLUME UPTAKE AND OUTPUT OF SUBSTANCES PERFUSED THROUGH LIVER SURVIVING IN AN ONCOMETER

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Abstract
Analyses of the inflow and outflow to a liver prep. surviving in an oncometer have been made for K, sugar and, in a few expts., also for chloride. The samples analyzed were collected from the hepatic outflow at frequent, successive and short-lasting intervals of time while the organ was responding to injs. of acetyl-[beta]-methylcholine chloride or adrenalin chloride into its inflow cannula. When the changes in vol. of the organ as well as changes in rate of outflow and inflow are taken into the calculations, then the amts. of uptake and output of substances contained in the perfusate may be quite different from those indicated by concs. alone at the sites of inflow and outflow. The differences in content per unit of time under certain conditions may be all out of proportion to the differences in content per unit of vol., and the values for the former may change greatly while those for the latter change not at all and vice versa. The method obviates resort to the procedure employed in chronic cases, namely, the taking of sections out of the organ under investigation. The method further makes possible the exptl. differentiation between the vascular and glandular effects of various agents upon the liver. The expts. here reported demonstrate this differentiating capacity of the method, especially when autonomomimetic agents are injected into the portal vein of the liver. The method promises further to be useful both in the study of the role played by an organ in producing transient alterations in the composition of the general circulation (minute-vol. output here is of the greatest importance); and in the study of the role played by the varying contents of the general circulation, or of affluent nutrient fluids, upon the organ itself in both its vascular and glandular responses.

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